Cell Types Electroporated

By the Axoporator 800A Single-Cell Electroporator

A sample of some of the cell types electroporated by Axoporator 800A, with lists of molecules successfully delivered to each cell type.

 
Aplysia Neurons

• Electroporation of neurons and growth cones in Aplysia californica.
  Peter Lovell, Sami H. Jezzini, and Leonid L. Moroz.
  Journal of Neuroscience Methods Online

Rat Hippocampal Pyramidal Neurons in Acute Slice

• FITC dextran 3000
• Tetramethylrhodamine dextran 3000

Mouse Cortex Neurons in Acute Slice

• FITC dextran 3000
• Tetramethylrhodamine dextran 3000

Rat Dorsal Root Ganglion Cells

• Alexa 488
• Alexa 568
• Clontech dsRed 1 mitochondrial clone
• Clontech eGFP peroxisome clone
• Dextran FITC 4,10,20,40,70,250

Aplysia Gastric Ganglia

• FITC dextran 3000

Rat Retinal Ganglion Cells

• Alexa 488
• Alexa 568
• Clontech dsRed 1 mitochondrial clone
• Clontech eGFP peroxisome clone
• Clontech eCFP nuclear clone
• Dextran FITC 4,10,20,40,70,250

Rat Photoreceptors

• Alexa 488
• Alexa 568

Zebrafish Tectal Neurons

• pEGFP

Alpha TN4 Cells (a mouse lens epithelium clonal cell line)

• Alexa 488
• Alexa 568
• Clontech dsRed 1 mitochondrial clone
• Clontech eGFP peroxisome clone
• Clontech eCFP nuclear clone
• Dextran FITC 4,10,20,40,70,250
• eGFP vimentin
• eGFP actin
• eGFP Kir2.1 inward rectifier clone
• eGFP Kv2.1 delayed rectifier clone
• eGFP Bk alpha subunit clone
• 40 eGFP partial sequence clones of Kir2.1
• CaCl2
• 2,4-Dinitrophenol
• Cytochalasin D
• eGFP, eCFP, eYFP or dsRed 1 clones of CD-20, adaptin, golgi, tubulin, endoplasmic reticulum, Lamin B, and lysosome
• siRNA's of several kinds

Rat Conjunctival Epithelium

• Alexa 488

Rat Ciliary Epithelium

• Alexa 488

Rat Lens Epithelium (collagenase treated to remove capsule)

• Alexa 488
• Alexa 568

Rat Corneal Endothelium

• Alexa 488
• Alexa 568

Rat Corneal Epithelium

• Alexa 488

Human Lens Epithelium (decapsulated prep)

• Alexa 488
• Alexa 568

Human Lens Epithelium (cultured)

• Alexa 488
• Clontech eCFP nucleus clone
• Clontech eYFP peroxisome clone
• Clontech dsRed1 mitochondrial clone
• Dextran FITC 4,10,20,40,70,250

Bovine Corneal Epithelium (cultured)

• Alexa 488
• Clontech eCFP nuclear clone
• Clontech eYFP peroxisome clone
• Clontech dsRed1 mitochondrial clone

Guinea Pig Interstitial Cells of Cajal

• Alexa 488
• Alexa 568
• Clontech dsRed1 mitochondrial clone
• Clontech eGFP peroxisome clone
• Dextran FITC 4,10,20,40,70,250