Analyst AD

Assay Development

 

multimode assay flexibility Analyst AD offers detection based on: Fluorescence intensity Fluorescence polarization Time-resolved fluorescence resonance energy transfer (TR-FRET) Luminescence Absorbance (epi- and trans-)

Analyst® AD Assay Development System is the only no-compromise, flexible system for the development of assays destined for High Throughput Screening (HTS). By sharing a common detection hardware and software system with Analyst HT (for HTS using 96- and 384-well formats), Analyst AD eliminates the need to modify and revalidate methods due to differences in detection system features and performance. Its high sensitivity and dynamic range in all modes also makes it ideal for basic research applications requiring fluorescence readout.

Analyst AD enables two-wavelength assays (such as FRET) or two-method assays. Analyst AD supports 96- and 384-well formats.

no-compromise performance

Analyst AD delivers uncompromising sensitivity and dynamic range in all fluorescence-based assay detection modes. Analyst AD achieves this performance with SmartOptics™, a patented system that selects and configures light sources, filters, detectors, and optical paths to ensure maximum assay performance. Using novel three-axis positioning, light is focused and collected in a small "sensed volume" for maximum performance, excluding spurious light that contributes to noise and cross-talk. The sensed volume is a small fraction of the total assay well volume and remains the same for 384-well microplates, ensuring essentially identical assay performance in 96- and 384-well formats—even with low volume plates such as HE- microplates.

complete method portability from assay development through HTS

All optical and detection settings are automated in Analyst AD using an intuitive graphical user interface called CriterionHost. Analyst AD is part of the Analyst line of instruments that include Analyst HT, Analyst GT (for ultra-HTS using the 1536-well format) and ScreenStation™ (for automated assay assembly and detection). These instruments share the SmartOptics hardware system. Any method developed on Analyst AD will have the same assay performance on the automation-ready Analyst HT. Minor modifications are required when transferring assays to Analyst GT due to its 1536-compatible optical elements.