SpectraMax 190

Microplate Reader with PatchCheck®

Features

• Use the extinction coefficient of your sample to calculate concentrations directly.
• Correct for volume differences in the wells.
• Expand the dynamic range of your assay.
• Detect pipetting errors.
• Check performance of your pipetting devices.

The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The corrected absorbance is within 5% of the value obtained in a conventional spectrophotometer.

Industry leading SoftMax® Pro microplate analysis software provides comprehensive instrument control and data reduction.

spectral scanning makes assay development easy

You can select the optimum wavelength for reading your assay by viewing the complete spectrum of your sample, allowing you to monitor the stability of your sample or reagent, determine interfering compounds or conditions of your assay, and validate your assay to regulatory standards.

accurate nucleic acid measurements

The SpectraMax 190 microplate spectrophotometer is the tool you need for DNA analysis. A bandwidth of 5 nm or less is required for accurate absorbance measurement of DNA¹. With the ability to detect DNA down to 25 ng per well, as well as a limit of calculation to 75 ng per well, the SpectraMax 190 microplate spectrophotometer gives you the same sensitivity as a conventional spectrophotometer.

expand the dynamic range of any assay to 0 to 6+ OD

The PathCheck sensor can extend the instrument linearity by a factor of three or more. For very concentrated samples, use a minimum volume in the microplate well (100 µl has a pathlength of approximately 0.30 cm), then use the PathCheck sensor to calculate an equivalent 1-cm pathlength absorbance value, giving you calculated absorbance values well beyond the capabilities of other instruments.

quickly determine multi-channel pipettor performance

Precision and accuracy of multi-channel pipettors can now be verified using the actual dispense reagent without the need to add a dye. By measuring the depth of the liquid in the well, the patented PathCheck sensor can determine the volume of the liquid dispensed². Even 96-channel dispensers can be tested in a matter of minutes.

early ADME compound profiling assays

SpectraMax microplate readers are ideal for increased throughput of early ADME compound profiling assays such as the Transil® Membrane Affinity Assay and Human Serum Albumin Binding Assay. The Membrane Affinity assay provides high-throughput lipophilicity data equivalent to liposome-based methods without the need for ultracentrifugation. The Human Serum Albumin Binding assay provides high-throughput blood protein binding data by measuring the fraction bound to Human Serum Albumin.

Specifications

photometric performance
Wavelength Range	190 - 850 nm
Wavelength Selection	Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth	2 nm
Wavelength Accuracy	<± 2.0 nm
Wavelength Repeatability	± 0.2 nm
Photometric Range	-0.3 to 4.0 OD
Photometric Resolution	0.001 OD
Photometric Accuracy (190-850 nm)	< ±0.006 OD ±1.0%, 0 - 2.0 OD
Photometric Precision (190-850 nm)	< ±0.003 OD ±1.0%, 0 - 2.0 OD
PathCheck Measurement Error	< 5% when compared to cuvette reading
Stray Light	< 0.05% @ 230 nm
Light Source	Xenon flash lamp
Microplate Read Time:    Endpoint    Kinetics	12 seconds 9 second minimum interval
Temperature Regulation Specifications
Temperature Range	Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate)	± 0.5 °C @ 37 °C, well-to-well
Temperature Stabilization Time	30 minutes maximum upon initiation
Reading Chambers	Isothermal when temperature regulation not enabled, <1 °C
physical specifications
Dimensions	22 cm (h) x 58 cm (w) x 38 cm (d)
Weight	30 lb (13.6 kg)

* US Patent Nos. 5,959,738 6,188,476 6,320,662 6,339,472 6,404,501, 6,496,260.