MetaMorph Image Display & Processing

MetaMorph offers a number of display and processing tools for binary and 8-, 16-, 24-, 48-bit images and stacks.

• Overlay or separate multiple wavelength images
• Color align images
• Create movies of time lapse or Z-series
• View multi-dimensional data sets in a dedicated viewer
• View orthogonal planes
• Stitch images
• Create intensity profiles
• Adjust contrast and scaling
• Add presentation graphics

4D Data Visualization

The new 4D Viewer facilitates the viewing of multi-dimensional data sets, stacks and sequential images. Users can simultaneously view multiple Z sections, wavelengths, time points and positions in a single intuitive viewing window, as well as binarize multidimensional image data into discrete objects, for 3D isosurface viewing and rotation. Isosurfaced objects selected by mouse are linked to an interactive spreadsheet of 3D object measurements, including volume, intensity, and several other measurement parameters.

Figure 1. A Z-series of a nerve stack is thresholded and isosurfaced to obtain volume measurements.

Image Enhancement

MetaMorph's processing functions may be used to enhance image characteristics that may not be discernable in the original image, making subsequent analysis and presentation more informative.

• Processing of images and stacks of images
• Binary operations such as invert, erode, and dilate
• 2D deconvolution (nearest neighbor and no neighbor)
• 3D deconvolution
• Basic filters such as sharpen, low pass, median filter, and remove haze
• Image and stack arithmetic
• Morphology filters such as erosion, dilation, open, close, and watershed segmentation
• Background subtraction and shading correction
• FFT (Fast Fourier Transform)
• Extract object boundaries for using edge detection

Morphology Filters

The Morphology Filters dialog box in MetaMorph offers over 20 operations enabling users to take advantage of a wider array of image processing and segmentation options for binary or grayscale images using state-of-the-art algorithms with an easy-to-use interface.

Common morphological filters include dilate, erode, open, close, top hat, detect/fill holes, detect/remove border objects, and more. Advanced operations and enhancements of standard filters typically only available in programming environments like Matlab™ are made easy with single-click options for filter shape selection, sequential filtering, morphological reconstruction, area-based filtering, H-dome, and watershed functions.

Additionally, filters enable advanced users to write more powerful macros called journals without the need for learning a programming language.

Read more about MetaMorph: acquisition, analysis, customization.