ImageXpress Velos Laser Scanning Cytometer

Fast-scan, whole-well imaging cytometry under 3 minutes

The ImageXpress® Velos Laser Scanning Cytometer is ideally suited for capturing rare events from a variety of heterogeneous samples including beads or cells, cell colonies, tissue samples, or even micro-array slides. In addition to multiplexing, the cytometer supports label-free light scattering and fluorescence anisotropy measurements. Image acquisition and data analysis occurs simultaneously at high speed to provide "on-the-fly" high throughput image analysis.

  • Fast and high throughput analysis of objects plated on any clear bottom microplate in <3 minutes.  With automation it can be configured to acquire in excess of 150,000 wells in an 8 hour day
  • Whole well scanning ensures sufficient number of objects for statistical validity.  Reduces the number of cells required as compared to either standard microplate readers or camera-based imagers.
  • Large depth of field (+/- 200 microns) eliminates focus steps during acquisition and provides easy imaging of large objects such as 3D cell cultures, mammospheres or zebrafish embryos.
  • Patented confined detection region reduces background from out of focus fluorescence and enables homogeneous / no-wash fluorescence assays like cell surface marker detection (e.g., E-cadherin), fluorescent ligand-receptor interactions, or antibody discovery.
  • Anisotropy detection mode enables solution and bead-based fluorescent polarization assays as well as simplified live cell FRET assays.  
  • Laser scatter mode for label-free detection of cells when intervening with dyes is not acceptable, for example in quality control of sterile cell cultures.
  • Microplate-based workflow streamlines evaluation of both adherent and suspension cells and eliminates separation steps for adherent cells or colonies often required for flow cytometry or other non-plate based systems.
  • On-the-fly analysis enables users to simultaneously acquire, count, and measure objects so that results are available at the end of each scan.
  • Robust, simple, and fast object detection means no microscopic imaging experience is required for setup making it easy for anyone in the lab to establish and run assays. 
  • Cell-by-cell and well-by-well results of object intensity, area, and shape are generated during each scan, allowing easy classification and gating of objects to streamline your work flow.
  • Output data in a variety of formats (.csv, .txt, .fcs) for easy downstream analysis and manipulation in a variety of software packages including flow cytometry software.
  • MDCStore™ Data Management Solution compatible files facilitate analysis with MetaXpress® for quantifying objects like zebrafish embryos and and AcuityXpress™ Software for data visualization and trend analysis.

 

Large Format Imaging

  • Use whole animal models such as zebrafish embryos or C. elegans. to study toxicology, developmental biology, or off-target drug effects 
  • Collect whole well, microscope slide, or petri-well plate images of large objects without repeated focusing, reconstructing z-slices, or stitching fields-of-view together
  • Analyze images during the scan for colony size or number or score organisms based on morphology or intensity 
Zebrafish embryos expressing GFP in
blood vessels were imaged in both
scatter and fluorescence mode. Embryos
were dispensed into 384-well plates
for toxicity testing. High speed acquisition
with on-the-fly analysis of whole well
images opens up a multitude of applications. 
Single image of a well in a 96-well plate
containing a monoclonal cell colony
stained with a live-cell fluorescent stain.

 

 

Further Information

Image-based Cytometry

  • Count and classify a large population of objects from an acquired image using a flow cytometry-type analysis
  • Analyze adherent or non-adherent cells in a 96- or 384-well plate format
  • Export resulting files for plotting and categorization in a standard flow cytometry software or an enhanced image-based cytometry package
The combination SCF, Flk3, TPO caused
differentiation of a greater number of
erythroid (CD34+) cells. 
SCF, IL-3, GM-CSF promoted differentiation
of cells toward (CD15+) myeloid cell phenotype.
Percent and numbers of CD34+ or CD15+
cells were measured on the cytometer.

Further Information

  • High-Throughput Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity: Poster Download

Screening

  • Measure apoptosis, cytotoxicity, mitotic arrest, DNA damage, phosphorylation, or ligand binding events
  • Detect up to four fluorescent markers within a primary or cultured immortalized cell line
  • Obtain whole-well imaging results with on-the-fly analysis in less than 3 minutes per microplate
Two color ratiometric assay for
mitotic index in 96 well plates.  
Chromium 488 (green) indicates
phospho-histone3 presence.  
Propidium Iodide (red) stains all cells.
96-well Oris Cell Migration Assay
from Platypus Technologies.
Image on the left is from well
with compound that inhibited migration
into the detection zone. Image on the right
is from a DMSO control treated well.
The assay works well with live or
fixed cells in both fluorescence or
scatter mode. Cells shown were fixed
and stained with propidium iodide.

Further Information

Homogeneous Assay

  • Screen antibodies using a homogeneous assays that measures the binding of a fluorescently labeled antibody to a bead or cell surface
  • Reject background fluorescence with angeled collection optics and ensure high reproducibility and sensitivity
  • Scan and analyze whole plate within 5-10 minutes regardless of well density
Homogenous bead assay performed
in 384-well plate.  Left well shows detection
of low concentration of primary antibody.
Right shows a well with high concentration
of primary antibody.  Fluorescent secondary
antibody is same concentration in all
wells and not removed.  Sensitivity is equivalent
to the ABI 8200 Cellular Detection System (FMAT).

Further Information

Fluorescence polarization or anisotropy

  • Measure enzymatic phosphorylation, single nucleotide polymorphisms (SNPs), and many ligand-receptor binding events
  • Provide cell-by-cell detail not found in microplate reader applications
  • Scan and analyze an anisotropy assay with up to 2 different fluorophores per well on-the-fly in 2-5 minutes 

Further Information

Basic Software

The ImageXpress Velos Cytometer includes intuitive software for acquisition and simultaneous analysis.

Customized Analysis and Data Management

Images can be imported into MDCStore™ Data Management Solution and analyzed with MetaXpress® or MetaXpress® PowerCore™ Software. Resulting data can be summarized and plotted using AcuityXpress™ Software.

FCS Express 4 Image Cytometry Software

This data analysis and reporting package designed by De Novo Software provides dynamic insight to multi-parametric data sets, enabling identification of common or rare events within large cell populations. Fully interactive tools improve the image analysis workflow and user's ability to manage results. 

 

The ImageXpress Velos SL Cytometer is a single-laser system configured from a selection of laser lines offered. The ImageXpress Velos DL Cytometer is a dual-line laser system consisting of a 488 nm laser system plus one additional laser line from the selection of laser lines offered. 

Excitation Wavelengths Available and Selectable Through Software

  • 405 nm
  • 440 nm
  • 488 nm
  • 532 nm
  • 640 nm

Software Selectable Detection Modes

  • Fluorescence
    • 4 color simultaneous
  • Anisotropy / Fluorescence Polarization
    • 2 color simultaneous
  • Laser scatter
    • Label-free imaging

Space and Weight Specifications

W (in) D (in) H (in) Weight (lbs) W (cm) D (cm) H (cm) Weight (kg)
18.5 29 15 140 47 73.7 38.1 59

 

For product support on the ImageXpress Velos System, please refer to the instrument manual or contact us.