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MetaFluor Fluorescence Ratio Imaging Software

MetaFluor® Fluorescence Ratio Imaging Software is the industry standard for monitoring fluorescence ratio imaging applications, bringing microscopists closer to rapid cellular responses inherent to live cells.

MetaFluor Fluorescence Ratio Imaging Software is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data; ratio image; graphs of intensities, ratios, and ion concentrations (e.g., calcium, sodium, potassium, chloride); and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities.


MetaFluor Fluorescence Ratio Imaging Software features:

  • Real time processing significantly reduces random image noise by averaging up to 256 images per time point. Background subtraction is also used to improve accuracy by correcting for stray light, camera noise, and auto-fluorescence.
  • Calibrations of intracellular ion concentrations are obtained by using the various calibration options offered: the Grynkiewicz equation (Grynkiewicz et al., 1985) and titration equation for both in situ and in vitro experiments. These calibrations can then be stored for future use.
  • Region analysis can be generated automatically or manually to monitor intensity, ratio value, or ion concentration. Measurements are then made simultaneously on all the regions of interest and update continuously on a scrolling graph, allowing you to follow dynamic changes as they occur in your living samples.
  • Interactive graphs give flexibility in the presentation of your experiment's data. MetaFluor Software enables you to click on graph traces to display a readout of the time and data value for the region nearest to the click.
  • Event mark function is useful to record when drugs or solutions are added, experimental conditions changed, triggers are received or sent, or other events occurred. You have the option to associate a timer and an alarm bell to each event. Additionally, for perfused samples, ambient conditions can be logged and tracked.
  • Journals allow you to create functions to simplify system operations, automate acquisition and device control, set variables, and sequence events. The Auto-Execute Journal command allows you to automatically run journals at specific points in the acquisition cycle.

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