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AcuityXpress Software for High Content Data Visualization and Analysis

Interpret high content screening data with high end data analysis and visualizations tools.

Making sense of High Content Screening data is usually a time-consuming and frustrating of endeavor because commercial informatics tools are not designed for HCS and often need customization. Sifting through large amounts of data requires bioinformatics groups with expertise in multi-parametric analysis tools. In addition, proper methods for quality control are frequently lacking, making it difficult to compare data from multiple conditions or to relate it to the initial image and ensure image acquisition and analysis were properly done. The AcuityXpress Software solves both problems by elegantly packaging easy-to-use high-end informatics tools with a seamless integration to the HCS workflow.


Full integration into the HCS workflow achieved by sharing the MDCStore™ Data Management Solution with the MetaXpress® Software.

A wizard-like interface to create advanced database queries and to compare multiple experimental conditions side-by-side.    

Bi-directional interactive image viewer brings visualization of HCS data to the next level, facilitating data quality control through direct interaction between image and numerical data, in both directions.

 

Play Video Play an example of "Drill-down" from plots to images.
Play Video Play an example of "Drill-Up" from images to curve-fitting.

Play Video Play an example of "drill-down" from a Principal Components Analysis to an image.
Play Video Play an example of "drill-up" from a single cell to a Self-Organized Map of all the cells in an experiment.

Multi-parametric analysis and visualization tools are provided alongside standard tools such as curve fitting and scatter plots. From K-Means, K-Medians, and hierarchical clustering to self-organizing maps and more, AcuityXpress Software reduces data complexity by automatically organizing phenotypes (responses to experimental conditions).

 

Multi-parametric analysis tools are provided alongside standard visualizations such as scatter plots to take full advantage of the multi-parametric nature of HCS data. (Top) 2D scatter plots. (Middle) Self-Organizing Maps automatically group similar biological responses together and help identify "hits" as well as unexpected trends such as QC issues or new responses that could be of interest for further exploration. (Bottom) Principal Components Analysis reduces the complexity of multi-parametric data to principal components amenable to 3D visualizations in which similar responses are grouped together.

Dose response analysis and curve fitting to generate both potency measurements (EC50/IC50) with predefined or custom functions and go beyond curve fitting and automatically organize dose responses based on the "shape" of the response (level of activity, slope, etc.) and get more insight into the data.

AcuityXpress Software offers curve-fitting to determine EC50/IC50 values from dose responses (left) as well as methods to further differenciate responses and automatically organize them based on the similarity of their shapes (right)

Data management and annotation tools assign experimental conditions (compound names, concentrations, and groups) to the images to ensure data is identified correctly. In addition, the "quicklist" feature tracks compounds or conditions of interests in multiple screens or data-views. A full list of data management and annotations is available on the "Specifications" tab.

Training Tools with course offerings at our Molecular Devices locations, customer sites, or online with the AcuityXpress Software Learning Center (access requires login) are available to make sure you can take full advantage of the AcuityXpress Software and make meaningful decisions from your high content data.