Microplate Readers
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Comparison of Microplate Readers with Luminescence Detection

Features

SpectraMax

Flex-
Station 3

M3

M4

M5

M5e

L 

Detector

Photo-multiplier tube

Photo-multiplier

tube

Photo-multiplier

tube

Photo-multiplier

tube

1, 2, or 6 Ultra-fast Photomultiplier Tubes

Photo-multiplier

tube

Spectral range

250-850

250-850

250-850

250-850

380-630 nm

250-850

nm

nm

nm

nm

 

nm

Sensitivity

<2 fg/well for firefly luciferase in 96- and 384- well top read

<2 fg/well for firefly luciferase in 96- and 384- well top read

<2 fg/well for firefly luciferase in 96- and 384- well top read

<2 fg/well for firefly luciferase in 96- and 384- well top read

20 attomol ATP per well, 0.2 fg firefly luciferase per well

<2 fg/well for firefly luciferase in 96- and 384- well top read

Dynamic range

 

 

 

 

> 9 decades

 

Microplate format

6-384

6-384

6-384

6-384

96- and 384-well microplates

6-384 (96- and 384-well with pipetting)

Cross-talk

<0.3% in white 96- and 384-well microplates

<0.3% in white 96- and 384-well microplates

<0.3% in white 96- and 384-well microplates

<0.3% in white 96- and 384-well microplates

< 3 x 10-5 (white plates)

<0.3% in white 96- and 384-well microplates

Read modes

Endpoint, kinetic

Endpoint, kinetic

Endpoint, kinetic

Endpoint, kinetic

Endpoint, dual read, kinetic, fast kinetic

Endpoint, kinetic, fast kinetic

Read times

0.1 up to 10 sec. integration time per well

0.1 up to 10 sec. integration time per well

0.1 up to 10 sec. integration time per well

0.1 up to 10 sec. integration time per well

0.1 up to 3600 sec. in steps of 0.1 sec.

0.1 up to 10 sec. integration time per well

Injectors/Pipettors

N/A

N/A

N/A

N/A

2, 4, or 12 injectors

8- or 16-channel pipettor

Injection/pipettor volumes

N/A

N/A

N/A

N/A

Selectable between 10 and 100 µl

Selectable between 5-200 µL (96-well) and 1-25 µL (384)

Temperature control

X

X

X

X

X

X

Software

brand

SoftMax Pro

SoftMax Pro

SoftMax Pro

SoftMax Pro

SoftMax Pro

SoftMax Pro

Automatic waste disposal

 

 

 

 

X

 

Robotic/automation

X

X

X

X

X

X

Validation & compliance tools

X

X

X

X

X

X

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