Microplate Readers
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Comparison of Microplate Readers with Fluorescence Detection

Feature

SpectraMax

FLIPR Tetra

Gemini

Flex-Station 3

M5e

M5

M4

M3

M2e

M2

XPS

EM

Fluorescence

x

x

x

x

x

x

x

x

x

x

Absorbance

x

x

x

x

x

x

 

 

 

x

Luminescence

x

x

x

x

x*

x*

x

x

x

x

Time-resolved

fluorescence (TRF)

x

x

x

 

x*

x*

 

x

x

x

HTRF Certified

x

 

 

 

 

 

 

 

 

x

Fluorescence Polarization

x

x

 

 

 

 

 

 

 

x

Wavelength range

FL 250 - 850, Abs 200 - 1000

FL 250 - 850 ex/em, Abs 200 - 1000

FL 250 - 850, Abs 200 - 1000

FL 250 - 850, Abs 200 - 1000

FL 250-850 ex, 250-850 em; Abs 200-1000

FL 250-850 ex, 360-850 em; Abs 200-1000

LEDs available in 360-626 nm range, Emission filters available in 400-706 nm range

FL 250-850 ex, 360-850 em

FL 250-850 ex, 250-850 em

FL 250 - 850, Abs 200 - 1000

Bandwidth

9/15

9/15

9/15

9/15

9/9

9/9

Variable

9/9

9/9

9/15

Tunable wavelength selection/ monochromator

x

x

x

x

x

X

 

x

x

x

Endpoint

x

x

x

x

x

X

 

x

x

x

Kinetic

x

x

x

x

x

X

x

x

x

x

Spectral scanning

x

x

x

x

x

X

 

x

x

x

Well scanning

x

x

x

x

x

X

 

x

x

x

Reading speed (sec)

17

17

17

17

15

15

1

15

15

17

Microplate formats

6-384

6-384

6-384

6-384

6-384

6-384

96, 384, 1536

6-384

6-384

6-384**

Integrated multi-

channel pipettor

 

 

 

 

 

 

 

 

 

x***

Temperature control

x

x

x

x

x

x

x

x

x

x

Top reading

x

x

x

x

x

x

 

x

x

x

Bottom reading

x

x

x

x

 

x

x

 

x

x

Cuvette port

x

x

x

x

x

x

 

 

 

x

Validation tools

x

x

x

x

x

x

 

x

x

x

Robotics / automation

x

x

x

x

x

x

x

x

x

x

All above trademarks are property of Molecular Devices, LLC.
* Secondary modes
**  96- and 384-well with pipetting
*** 8- and 16-channel pipettor