The SpectraMax i3 Multi-Mode Detection Platform is a microplate system that evolves with your future needs. The MiniMax Imaging Cytometer option is an upgradable component to the base system that offers cellular imaging capability, and much more. 

Simple workflow

• Cell and whole-well assays in a small footprint

More data

• Multi-parametric cell-based assays with brightfield and fluorescent modes

Familiar software

• Set up, image and analyze cells in a snap using SoftMax® Pro Software

• Use fewer and or less fluorescent cells compared to whole-well measurements

Lower variance

• Normalize readings with number of cells in a well

Cell count/viability

• CHO cells were plated in 2X dilutions starting from 32000 cells/well in a 96-well plate
• Cells were then stained with Calcein AM dye and counted

Top: The cell counting module of the SoftMax Pro Software MiniMax Imaging Edition displays segmentation mask of the image (left), selected cell-by-cell measurements (middle), and a histogram of objects by average intensity (right). Bottom: Software allows user to graph cell counts of serially diluted CHO cells.

Cell proliferation/cytotoxicity

• Hepatocytes were treated with known compounds causing liver toxicity
• Proliferation/toxicity effects assessed by measuring total cell area

Top: Heat map of hepatotixicity experiment demonstrating dose-wise response to four compounds (blue - low area cells; red - high area cells). Bottom: After image analysis, the software plots results and fits the curves to determine IC50s (red - aflatoxin 1; green - aflatoxin 4; blue - staurosporine; purple - mitomycin C). iCell® Hepatocytes from Cellular Dynamics International.

Marker expression

• HUVEC cells were pre-treated with anti-inflammatory compounds and then cultured in the presence of inflammatory cytokines
• Stimulated protein expression was measured by total integrated intensity

Top: Expression of VCAM protein on cells activated by inflammatory cytokines. Bottom: Total integrated intensity of adhesion molecules expressed in the presence of anti-inflammatory compounds (blue - SB202190; red - SB203580)

Technical specifications

The MiniMax Imaging Cytometer is a user-upgradable option to the SpectraMax i3 Multi-Mode Detection Platform. It can be purchased together with the SpectraMax i3 base system, or added separately should your application needs expand in the future. 

Part Numbers

• SpectraMax MiniMax Imaging Cytometer; PN: 5024062
• SpectraMax MiniMax Desktop Computer; PN: 5023982     
• SpectraMax MiniMax 22" Monitor; PN: 5024296
• SpectraMax i3 Multi-Mode Detection Platform; PN: i3