Key Benefits include:
- User-Upgradeability Expand the 3-mode base system with additional application cartridges for FP, HTRF®, AlphaScreen® assays, and cellular imaging
- SpectraMax® MiniMaxTM Imaging Cytometer Simplify complex imaging workflow by visualizing cell morphology changes on top of well-based intensity readings
- Spectral FusionTM Illumination Delivers high-powered full spectrum light source for increased fluorescence performance across the entire excitation range
- Expanded Dynamic Range Sophisticated engineering delivers optimal sensitivity and maximizes signal range
- SoftMax® Pro Microplate Data Acquisition & Analysis Software Industry-leading SoftMax Pro Software streamlines complex data analysis with over 140 built-in protocols, effectively eliminating the need for additional software. Additional tools for regulatory compliance are included in SoftMax Pro GxP Software
Base System Technical Specifications
| General Specifications |
| Dimensions (in) |
12.63 (H) x 15.38 (W) x 23.38 (D)
|
| Dimensions (cm) |
42.23 (H) x 39.05 (W) x 59.37 (D)
|
| Weight |
36 lbs. (16.4 kg)
|
| Power consumption |
< 200 watts
|
| Power source |
100-240 Vac, 2 A, 50/60 Hz
|
| Robotic-compatible |
Yes |
| General Photometric Performance |
Plate formats
|
6 to 1536 wells§
|
Light source
|
Spectral Fusion Illumination (Xenon flash lamp + high-powered LEDs or laser diode in detection cartridges)
|
Reading capabilities
|
Microplates, cuvettes (via adapter)
|
Detectors
|
PMT and/or photodiode
|
Shaking
|
Linear and orbital
|
| Injectors |
Option available |
Temperature control
|
4°C above ambient to 45°C |
Temperature uniformity
|
±0.75°C |
Temperature accuracy
|
±1°C at 37°C set point |
| Environmental control |
Gas quick connect |
Endpoint reading
|
All modes
|
Kinetic reading
|
All modes
|
Spectral scanning
|
Abs, Fl, Lum
|
Well scanning
|
Over 20 by 20 in all modes
|
Wavelength selection
|
1.0 nm increments
|
Standard Read Times (minutes:seconds)*
|
|
96 wells |
384 wells
|
Absorbance
|
0:30 |
1:40
|
Fluorescence Intensity
|
0:25 |
1:25
|
Luminescence
|
0:30 |
1:15
|
Absorbance Photometric Performance
|
Wavelength range
|
230-1000 nm |
Wavelength bandwidth
|
4.0 nm |
Wavelength accuracy
|
± 2.0 nm |
Wavelength repeatability
|
± 1.0 nm |
Photometric range
|
0-4.0 OD
|
Photometric resolution
|
0.001 OD
|
Photometric accuracy
|
< ± 0.010 OD ± 1.0%, 0-2 OD
|
Photometric precision
|
< ± 0.003 OD ± 1.0%, 0-2 OD
|
Stray light
|
< 0.05% @ 230 nm
|
Fluorescence Intensity Performance
|
Wavelength range
|
250-850 nm
|
Wavelength selection
|
1.0 nm increments
|
Bandwidth (EX, EM)
|
Adjustable
EX 9/15 nm | EM 15/25 nm
|
| Dynamic range |
> 6 logs
|
22
|
Optimized |
Guaranteed**
|
96 wells
|
0.5 pM
|
3 pM
|
384 wells
|
1 pM
|
4 pM
|
Bottom Sensitivity (Fluorescein)
|
96 wells
|
5 pM
|
10 pM
|
384 wells
|
5 pM
|
20 pM
|
Luminescence Performance
|
Wavelength range
|
300-850 nm
|
Wavelength selection
|
Choice of simultaneous detection of all wavelenths or selection in 1.0 nm increments
|
Dynamic range
|
> 6 decades
|
Cross-talk
|
< 0.3% in white 96- and 384-well microplates
|
Sensitivity (ATP-Glow)
|
Optimized |
Guaranteed**
|
96 wells
|
15 pM
|
75 pM
|
384 wells
|
30 pM
|
200 pM
|
* with 6 flashes in absorbance and 3 flashes in fluorescence mode, and 0.1 sec/well integration in 96-well luminescence mode and 0.04 sec/well integration in 384-well luminescence mode
§ 1536 detection available via detection cartridges
**For properly functioning, operating, and maintained equipment
The SpectraMax® MiniMax™ Imaging Cytometer option, user-upgradeable to any SpectraMax i3 base system, provides researchers with cellular analysis capability without the need for investment in complex imaging systems. The system adds rich, cell-by-cell statistics on top of well-based intensity readings, allowing for quick visual inspection of cells and multi-parametric assays.
Features:
- Brightfield and fluorescent imaging
- Cytotoxicity, protein expression and cell proliferation assays
- ANSI/SBS conformant plate compatibility
Benefits:
- Obtain cell-based data on top of well-based intensity readings in the same instrument
- Perform multi-parametric assays and get cell-by-cell statistics
- Use fewer and or less fluorescent cells compared to whole-well measurements
- Identify and segment cells quickly using SoftMax® Pro Software
With the SpectraMax i3 Multi-Mode Detection Platform, your creativity is unfettered. From biochemical to cell-based assays, there's no need to compromise your microplate detection needs. With numerous detection cartridges available, the SpectraMax® MiniMax™ Imaging Cytometer option for cellular imaging assays, along with SpectraDrop™ Micro Volume Microplate, applications are plentiful.
Representative Applications
- AlphaScreen®
- Apoptosis
- BRET
- Cellular imaging assays
- Cell proliferation, viability, cytotoxicity
- Enzyme activity
- Epigenetics
- FRET
- Genotyping
- GPCRs
- GTP binding
- Hormones
- HTRF®
- Immunoassays
- Ion channels
- Micro Volume (Low volume) Applications
- Molecular interactions
- Nuclear receptor
- Nucleic acid and Protein quantitation
- Reporter assay
- Time-Resolved Fluorescence (TRF)
SpectraMax i3 Multi-Mode Microplate Reader Platform
