Options for ImageXpress Micro High Content Screening System
A cornucopia of assays to monitor live cell biological processes are enabled with the platform’s modular design allows everything from simple label-free imaging to long monitoring of cellular responses to compound addition and post-wash recovery.
The unique phase contrast optics in the ImageXpress Micro System enables high contrast imaging of unstained samples to monitor biorelevant cell types (e.g. stem cell derived neurons) that may be overly sensitive to the effects of fluorescent labels or need to be monitored over time without intervention. This technique can also be used to scrutinize cell quality before and after biochemical assays, or combined with fluorescent applications and environmental control to examine multiple parameters at once. Click here to learn more about measuring neurite outgrowth completely label free.
When evaluating biological responses, tight control of the environment is one of the most critical factors in simulating physiological conditions and ensuring successful live-cell imaging experiments. The Live Cell or Environmental Control Option for the ImageXpress Micro System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high content screening kinetic and time lapse experiments a reality.
| Hela Cells Plated on 96-well plates and treated with 100 um Etoposide. Each well of plate was visited before returning to first well. Watch the 30 hour movie to see an increase in the number of dead and apoptosing cells. Blue = Hoechst for all Nuclei; Red = Propidium Iodide for Dead Cells; Green = Nucview488 a fluorescence substrate for Caspase 3 and an apoptosis sensor. Overlayed on a phase contrast image. |
Watch VideoDuring assay development, the Fluidics Option facilitates compound addition and cell washing to characterize compound potency or to determine incubation times for higher throughput fixed time point assays. On-board liquid handling can be combined with the External Filter Wheel Option to monitor dual-excitation wavelength intracellular ion concentration measurements (e.g. calcium, pH) to provide greater insight to ion exchange and intracellular function.
Transmitted Light Option with unique true phase contrast optics
Features
- Brightfield setting can be used with any objective lenses offered and is ideal for non-fluorescent histochemically stained samples.
- Phase optics enables label-free cellular imaging to be performed at a variety of magnifications (4x -60x; click here for objective compatibility chart). The unique offering of phase contrast optics on a high content screening system generates high contrast images where unstained cells are easily viewed or separated from their background. (Learn More) - Link to app module of Neurite outgrowth and phase contrast.
- Overlay fluorescent images on unstained phase images gives the user another fluorophore independent visualization of cell morphology
- Modular Design enables configuration at time of purchase or systems can be upgraded in the field at any time.
Specifications
- Nikon 100w Pillar DIASCOPIC ILLUMINATOR with TE-C ELWD Condenser
- The condenser is 0.3NA with 65 mm WD and PhL, Ph1, and Ph2 selectable phase rings
- Compatible with environmental control
- Not Compatible with Fluidics Option
- Click here for size and weight specifications
