Transmitted Light Options
The ImageXpress® Micro System enables contrast imaging of unstained samples to monitor cells that may be overly sensitive to the effects of fluorescent labels or need to be monitored over time without intervention. Two available options, Phase Contrast or Brighfield Only, can be used to scrutinize cell quality before and after biochemical assays, or combined with fluorescent applications and environmental control to examine multiple parameters at once.
Features
- Phase optics enables high contrast, label-free cellular imaging to be performed at a variety of magnifications (4x -60x).
- Brightfield only option can be used with any objective lenses offered and is ideal for non-fluorescent histochemically stained samples.
- Overlay fluorescent images on unstained images gives the user another fluorophore independent visualization of cell morphology
Specifications
- Nikon 100w Pillar Diascopic Illuminator with TE-C ELWD Condenser
- The condenser is 0.3NA with 65 mm WD and PhL, Ph1, and Ph2 selectable phase rings
- Phase Contrast option is compatible with Environmental Control, but not with Fluidics Option
- Brightfield Only option comes standard with Environmental Control and Fluidics Option
- Click here for size and weight specifications
Environmental Control Option
The Environmental Control Option for the ImageXpress Micro System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high content screening kinetic and time lapse experiments a reality.
Features
- User-defined premixed air allows researchers to select atmospheric conditions most appropriate for their cell type (e.g. 5% or 10% CO2).
- Temperature control of the sample plate helps maintain physiological environment.
- Humidity regulation is maintained above sample plate to minimize evaporation of media and extend measurement time.
- The high resolution stage synergizes with the Environmental Control Option of the ImageXpress Micro system to enable fast kinetic imaging of live cells or long time lapse monitoring of each well in a plate for hours or days (link to Furuyama-san movie), Link to Movie of zebrafish heart beat.
Specifications
- Accepts pressurized air from premixed tanks
- Temperature maintained in top half of instrument: 30-40°C ± 0.5°C
- Temperature measured near the sample plate is recorded and stored with each image in software
- Humidity maintained above plate; evaporation < 0.5 μL/well/hour (96- or 384-well formats)
- Compatible with Fluidics or Phase Contrast Transmitted Light options
- Click here for size and weight specifications
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 Watch Video
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Hela Cells Plated on 96-well plates and treated with 100 um Etoposide. Each well of plate was visited before returning to first well. Watch the 30 hour movie to see an increase in the number of dead and apoptosing cells. Blue = Hoechst for all Nuclei; Red = Propidium Iodide for Dead Cells; Green = Nucview488 a fluorescence substrate for Caspase 3 and an apoptosis sensor. Overlayed on a phase contrast image.
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Fluidics Option
During assay development, the Fluidics Option facilitates compound addition and cell washing to characterize compound potency or to determine incubation times for higher throughput fixed time point assays.
Features
- Configurable pipettor can accommodate 96- or 384-well pipette tips, compound plates and sample plates giving you the flexibility to change assay formats when you wish.
- Tip exchange option minimizes cross contamination often seen with non-contact, piezo- or pin tool-based fluidics systems
- Integrated pipettor (3-200µl) facilitates media replenishment or compound addition to individual wells within a microplate. Media replenishment can extend the time frame of long time lapse experiments and overcome any evaporation that may occur over time.
- External Filter Wheel option can be combined with liquid handling to monitor dual-excitation wavelength intracellular ion concentration measurements (e.g. calcium, pH) to provide greater insight to ion exchange and intracellular function.
Specifications
- Single-channel pipetting
- Dispenses volumes from 3 μL to 200 μL
- ±1 from 3uL to 20uL ±5% from 20uL to 200uL
- Holds two compound or media plates with optional heating
- 96- or 384-well format FLIPR® tips
- Comes standard with Environmental Control option.
- Compatible with Brightfield Only Transmitted Light option but not with Phase Contrast Transmitted Light Option.
- Click here for size and weight specifications
External Excitation Filter Wheel Option
Features
- 10 position excitation filter wheel increases the number of preconfigured filters installed with system to support multi-pass dichroic and emission filters in core facilities with a large variety of fluorophores in use.
- Fast filter exchange is ideal for exciting ratiometric indicators (e.g. Fura-2; BCECF) to monitor fast cellular responses and real-time kinetics indicative of ion exchange and intracellular signaling.
Specifications
- Controller drives one filter wheel
- Wheel can hold up to 10 excitation filters
- Switching times of up to 40msec between adjacent filters
- User adjustable speed allows the speed to load matching
- Wheel is inserted into the light source so additional space is not required
Light Source Option
Features
- Broad spectrum xenon light source is suitable for most fluorescent applications, compatible with filterwheel for ratio imaging (e.g. Fura-2; BCECF) and Multi-passexcitation cubes for additional flexibility
- Lifetime: 500+ hrs. Wavelength: 340-680 nm
- Included in ImageXpress Micro Standard Model.
- Solid state white-light engine with long lifetime, suitable for mostfluorescent applications
- Lifetime: 10,000+ hrs. Wavelength: 38o-680 nm
- Included in ImageXpress Micro XL Model
- Broad spectrum long-life light source is suitable for most fluorescent applications, 10 position filter wheel included for ratio imaging (Fura-2; BCECF). Compatible with multi-pass excitation cubes for additional flexibility and speed.
- Lifetime: 10,000+ hrs. Wavelength: 340-680 nm
- Optional upgrade for ImageXpress Standard and XL model.
- Near-IR optimized solid state whitelight engine with long lifetime, suitable for most fluorescent applications enhanced for near-IR application like Cy7
- Lifetime: 10,000+ hrs. Wavelength: 380-770 nm
- Optional upgrade for ImageXpress Standard and XL model
- Configurable solid state light engine is a customizable excitation source with independent control of up to 7 wavelengths. It expands flexibility of wavelength selection and is compatible with multi-pass excitation cubes for additional throughput and speed.
- Lifetime: 10,000+ hrs. Wavelength: Customizable 380 nm to NIR
- Optional upgrade for ImageXpress Standard and XL model
Options for ImageXpress Micro High Content Screening System