Neurotransmitter Transporter Uptake Assay Kit
Fluorescence-based assay for the detection of serotonin, norepinephrine, and dopamine transporter (SERT, NET and DAT, respectively) activity in expressing cells, transfected or primary
Neurotransmitter transporters play a key role in depression and neurodegenerative diseases (NDD) such as Alzheimer's and Parkinson's. The ability to monitor serotonin, norepinephrine and dopamine neurotransmitter uptake is key to a better understanding of these diseases.
Until recently, radioactively labeled compounds were used to measure serotonin, norepinephrine and dopamine transporter uptake. With the introduction of the Neurotransmitter Transporter Uptake Assay Kit, researchers now have a tool to screen for live-cell kinetic uptake for these three key neurotransmitters.
- Homogeneous, live-cell fluorescent assay replaces radioactive assays, reducing disposal costs and eliminating safety risks associated with radiolabeled assays
- One-step, homogeneous assay format is amenable to automation and high-throughput screening
- Two assay setup options offer greater flexibility: endpoint mode for high-throughput applications, or real-time kinetic mode for mechanistic studies
This assay utilizes a Molecular Devices masking dye to extinguish extracellular fluorescence. This masking dye technology has been exclusively licensed from Bayer AG (U.S. Patent Nos. 6,420,183, 7,063,952, 7,138,280 and European Patent No. 0,906,572). The assay also includes a novel proprietary fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine, and is actively transported into the cells via the neurotransmitter transporters.