Neurotransmitter Transporter Assay
The homogeneous Neurotransmitter Transporter Uptake Assay Kit provides a fast, simple, and reliable fluorescence-based assay for the detection of dopamine, norepinephrine, and serotonin transporter (DAT, NET and SERT, respectively) activity in expressing cells, transfected or primary.
Neurotransmitter transporters have become important pharmaceutical targets, as they play a key role in depression and neurodegenerative diseases (NDD) such as Alzheimer's and Parkinson's diseases. The ability to monitor serotonin, norepinephrine and dopamine neurotransmitter uptake is key to a better understanding of these diseases.
Until recently, radioactively labeled compounds were used to measure serotonin, norepinephrine, and dopamine transporter uptake. With the introduction of this novel assay kit, researchers now have a tool to screen for live-cell kinetic uptake for these three key neurotransmitters in a homogeneous, fluorescence-based, high-throughput format. The assay can be performed either as a real-time kinetic assay suitable for mechanistic studies or as an HTS-amenable endpoint read.
Benefits of the Neurotransmitter Transporter Uptake Assay Kit
- Homogeneous, live-cell fluorescent assay replaces radioactive assays, reducing disposal costs and eliminating safety risks associated with radiolabeled assays.
- One-step, homogeneous assay format is amenable to automation and high-throughput screening.
- Two assay setup options offer greater flexibility: endpoint mode for high-throughput applications, or real-time kinetic mode for mechanistic studies.
This assay utilizes a Molecular Devices masking dye to extinguish extracellular fluorescence. This masking dye technology has been exclusively licensed from Bayer AG (U.S. Patent Nos. 6,420,183, 7,063,952, 7,138,280 and European Patent No. 0,906,572). The assay also includes a novel proprietary fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine, and is actively transported into the cells via the neurotransmitter transporters.
The Neurotransmitter Transporter Uptake Assay Kit uses a fluorescent substrate that mimics the biogenic amine neurotransmitters and is taken into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity that is monitored in real time using a bottom-reading microplate reader. In this convenient no-wash procedure, cells are incubated with the kit reagent and transferred to the plate reader for evaluation. The assay is ideal for use in academic, biotech, and pharmaceutical environments for research, assay development, and screening.
For more details, please refer to the Neurotransmitter Transporter Uptake Assay Kit product insert, which may currently be accessed through the Customer Portal.