QBT Fatty Acid Uptake Assay Kit
Single-step, homogeneous, fluorescent assay for monitoring the activity of fatty acid transport proteins
The QBT™ Fatty Acid Uptake Assay is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.
Conventional protocols for monitoring fatty acid uptake using radioactivity often require cell lysis and processing at very low temperature, making them expensive, slow and unsuitable for high throughput screening. Other fluorescence-based protocols generally require the use of low throughput fluorescence activated cell sorter (FACS) instrumentation, or require cell washing, which can severely compromise the integrity of the fragile adipocytes used in these assays.
The QBT Fatty Acid Uptake Assay uses a BODIPY® -dodecanoic acid fluorescent fatty acid analog coupled with Molecular Devices proprietary quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids and is a known substrate for fatty acid transporters.
- One-step, homogeneous assay format enables high-throughput screening
- Fluorescent assay with proprietary quench technology allows users to monitor real-time uptake kinetics in live cells
- Replaces cumbersome and time-consuming fluorescent and radioactive assay protocols
- The elimination of radioactive compounds reduces disposal costs and eliminates safety risks associated with radioactive assays