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ScanLater Western Blot Assay KitScanLater Western Blot Assay Kit

Substrate-free immunoblot assay for extended signal stability

Pre-optimized for the ScanLater™ Western Blot Detection Cartridge, this assay kit enables the first western blot detection capability in a microplate reader. Utilizing this substrate-free assay, researchers can achieve equivalent sensitivity to traditional chemiluminescence and fluorescence-based assays, while consolidating western blot and ELISA applications onto a single reader.


The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. Using this novel western blot method, researchers can:

  • Eliminate time-dependent substrate addition steps
  • Sustain blot signal stability for at least one month
  • Enhance assay sensitivity using TRF method to reduce background noise
  • Maintain femtogram to picogram protein sensitivity similar to traditional western blot detectors

Features

  • Time-resolved fluorescence detection
  • Europium-labeled goat anti-rabbit, anti-mouse or streptavidin labeled antibodies
  • Supports nitrocellulose and PVDF membranes
  • 340/80 nm excitation with 616/10 nm emission range
  • Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3 and Paradigm® Multi-Mode Platforms