FLIPR Membrane Potential Assay Kits
Mix-and-read simplicity to rapidly screen ion channel targets
The FLIPR® Membrane Potential Assay Kit enables observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins. Each homogeneous assay kit utilizes a proprietary indicator dye and quencher combination to maximize cell line/channel/compound applicability, while eliminating causes of data variability. This unique formula responds 10 times faster and has greater temperature stability than traditional dyes, providing high quality screening data that shows good correlation with manual patch clamp assays.
FLIPR Membrane Potential Assay Kits detect ion channel modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. The fluorescent signal increases in intensity during membrane depolarization as dye follows the positively charged ions inside the cell. During membrane hyperpolarization, fluorescent signal decreases in intensity as dye follows the positively charged ions out of the cell. FLIPR Membrane Potential Assay Kits are uniquely suited for use with the simultaneous pipet and read capability of the FLIPR® Tetra System and FlexStation® Microplate Reader to capture fast kinetics associated with ion channel activation.