CatchPoint Cyclic-AMP Fluorescent Assay Kit
Measures cAMP levels via a competitive immunoassay format offering high sensitivity and flexible readout times
Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.
The CatchPoint® Cyclic-AMP Fluorescent Assay Kit's high-affinity reagents are optimized for sensitivity and precision in applications where cAMP levels are low. A single wash step removes unbound material prior to the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate a stable fluorescent readout, allowing the assay to be read in as little as 10 minutes or up to 24 hours after substrate addition.
Benefits of the CatchPoint cAMP Fluorescent Assay Kit
- High affinity reagents are optimized for sensitivity when cAMP levels are low, ensuring results when other assays fail
- Robust format resists interference from colored or fluorescent compounds, improving reliability of results
- Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening
- Quantitative method allows accurate determination of cAMP in samples