Webinar: Secrets for Improving GPCR Binding and Functional Assessment
Wednesday, July 13, 2011 8:00am - 9:00amThe recording from the webinar on Secrets to Improving GPCR Binding and Functional Assessment is available now.
Highlights:
- Expand Receptor Testing To Functional Readout including Second Messager and Kinase Pathway assessment
- Utilizing Receptor Binding Data To Understand Mechanism of Action
- Investigation of cell surface receptors and GPCRs with Tag-lite® homogeneous fluorescence technology platform
- Characterization of GPCR agonists and antagonists in a high-throughput assay system using SpectraMax® plate readers
Presenters:
- Francois Degorce, Director Marketing, Cisbio Bioassays
- Oksana Sirenko, Research Scientist, Molecular Devices, Inc
- Yvonne Beirne FitzGerald, Field Application Scientist, Molecular Devices, Inc
Information:
We will describe methods and present specific binding data for characterization of agonists and antagonists to several GPCR receptors including Mu Opioid, Dopamine D3, and Glucagon GLP1 receptors. We will also describe and demonstrate performance of several other functional assays using HTRF® technology: cAMP detection, p-ERK and p-AKT kinase assays. Studies will focusing on ligand binding and numerous functionality readouts using the same assay platform.
The Tag-lite® cellular platform is ideal to screen cell surface receptor targets, in particular GPCRs. It provides a wide range of assay formats for the investigation of receptor ligand binding and function. The Paradigm plate reader platform with a HTRF® cartridge provides fast, sensitive readout of the binding assay. The combination of the SpectraMax Paradigm® plate reader with the HTRF® and Tag-lite® suite of assays is shown to be a powerful assay platform for cell surface binding and functional assays and well suited for high throughput screening.
Please check back regularly for information on upcoming events, including user meetings and web seminars. Or contact Yvonne.fitzgerald@moldev.com if you have questions.