Getting Started Analyzing Molecular Interactions with AlphaScreen, a Webinar featuring SpectraMax Paradigm

The recording for Getting Started Analyzing Molecular Interactions with AlphaScreen, a webinar featuring SpectraMax Paradigm is available now.

Subject:

AlphaScreen^®: Analyzing Molecular Interactions with the SpectraMax^® Paradigm^® Multi-Mode Microplate Reader

Topics to be covered include:

• Alpha Technology: A Basic Introduction
• Applications for Alpha Technology
• An Endothelial Based In Vitro Model for the Detection of Anti-Inflammatory Metabolites from Cyanobacteria  Using AlphaLisa^TM

Presenters:

• Harald Hundsberger, Professor (FH) Ph D., University of Applied Sciences Krems, Austria
• Cathy Olsen, Ph.D., Application Scientist, Molecular Devices, Inc.
• Yvonne Beirne FitzGerald, Field Application Scientist, Molecular Devices, Inc 

Details:

Alpha Technology from PerkinElmer is a bead-based, homogeneous assay for studying molecular interactions in a microplate format. Binding of molecules captured on donor and acceptor beads leads to an energy transfer that results in a luminescent signal.  Latex-based beads allow users to immobilize a wide range of biomolecules in a modular manner and design a customized molecular interaction assay.  AlphaLisa^TM beads also enable one to run homogeneous, miniaturized ELISAs without the need for washing.  Alpha Technology can be performed on Molecular Devices' SpectraMax^® Paradigm^® Multi-Mode Microplate Reader in various plate formats ranging from 96-well to 1536-well.

This webinar will introduce you to Alpha Technology and the wide range of applications it enables, including oncology, immunology, and biochemistry.  We will also describe how AlphaScreen^TM assays have been used to detect anti-inflammatory metabolites from cyanobacteria in human endothelial cell-based in vitro assays.  Inflammation is accompanied by an increased endothelial chemokine production and adhesion molecule expression, which may result in an extensive neutrophil infiltration. As such, the search for novel anti-inflammatory substances, able to downregulate these parameters as well as the tissue damage hold therapeutic promise.  We show the quantification of cytokines (TNF alpha) down to picogram levels with the SpectraMax^® Paradigm^® Modular Multi-Mode Reader.

Please check back regularly for information on upcoming events, including user meetings and web seminars. Or contact Yvonne.fitzgerald@moldev.com if you have questions.