Neurotransmitter transporters are important pharmaceutical targets as they play a key role in psychiatric and neurodegenerative diseases as well as seizure disorders, addition, learning, and memory. To date, more than 20 members of the neurotransmitter transporter family have been identified. These include cell surface reuptake mechanisms for monoamine and amino acid neurotransmitters as well as vesicular transporter mechanisms involved in neurotransmitter storage. The ability to monitor the uptake of serotonin, norepinephrine, dopamine, glutamate, gamma amino butyric acid, and other neurotransmitters is key to a better understanding of these and other diseases.
Until recently, radioactively-labeled compounds were used to measure transporter uptake. Today, researchers can leverage a large arsenal of fluorescent transporter uptake assays that enable safer and higher-throughput screening of live cells.
Uptake transporters for fatty acids are also a critical target for drug discovery. Fatty Acids are used for diverse cellular processes including mitochondria oxidation, membrane synthesis, and energy storage. Increases in intracellular fatty acid concentrations can cause apoptosis and have been linked to insulin desensitization, Type 2 diabetes, obesity, and cardiovascular disease.
Fatty acid transport proteins (FATPs) are part of an evolutionarily conserved family, with six members expressed in the human genome. To better understand the role that FATPs play in disease, researchers need a robust assay that can quantify fatty acid uptake. Conventional radioactive protocols are expensive, slow, and not suitable for high-throughput screening, and newer, fluorescence-based approaches are often less than ideal as they require low-throughput flow cytometry, or cell washing which can compromise the integrity of adipocytes.
Recognizing the value of studying neurotransmitter and fatty acid uptake transporters in the context of disease conditions such as cardiovascular and metabolic disorders, Molecular Devices has developed unique fatty acid and neurotrasmitter uptake assays that enable researchers to delve deeper into the biological functions of these critical discovery targets. Please use the links below to learn more.
Content: Our systems address all content needs from low to mid to high content analysis & screening.