High Throughput Screening
High throughput screening (HTS) remains a critical step in the discovery of lead chemical structures for novel drug targets. To increase the probability of finding new leads from HTS, many labs have invested heavily in expanding both the diversity and quality of compound libraries.
A primary screen is designed to rapidly identify hits from compound libraries. The goals are to minimize the number of false positives and maximize the number of confirmed hits. Depending on the assay, hit rates typically range between 0.1 and 5 percent.
Typically, primary screens are run in multiplets (i.e., two, three, or more assay data points) of single compound concentrations. Readouts are expressed as percent activity in comparison to a positive (100 percent) and a negative (0 percent) control. Hits are then retested, usually independently from the first assay. If a compound exhibits the same activity, it is termed a confirmed hit, which proceeds to secondary screens or lead optimization.
Molecular Devices provides several screening platforms to support high-throughput screening for ion channel, GPCR, kinase, and transporter target classes as well as others. Please select among the links below to learn how we can help drive the success of your drug development, bioanalytical, and life science research lead identification efforts.
Throughput: We have the right system for your lab--from single-readout instruments to automated, multi-detection systems.
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