High Content Analysis

High content analysis (HCA) reflects the convergence of cell-based assays, high-resolution imaging, and advanced image processing and analysis. It addresses many challenges of traditional techniques, which can be destructive, time-consuming, and limited in the number and complexity of assays that can be performed. By employing image-based cellular assays in high-throughput formats, HCA enables researchers to increase the number of questions they can ask while simultaneously decreasing time to result.

High Content Analysis or HCA permits temporal and subcellular spatial tracking of proteins in living cells. In addition, it provides multiplexed readouts that help researchers integrate responses of multiple cellular targets and processes, and allows researchers to work with physiologically-relevant cell types.

Further, an ever-increasing selection of analytical tools that improve multi-parameter analysis, data management, and visualization methods is helping researchers manage the large number of data points generated by HCA more easily and effectively.

While generally ideal for cellular analyses, HCA has demonstrated particular value to researchers interested in studying interfering RNA (RNAi), neurons, stem cells, and cytotoxicity.

RNAi is an excellent tool for gene expression profiling, providing information about loss of function on a genome-wide scale. The technique helps researchers gain a deeper understanding of disease-associated pathways.

Neurons are difficult to target, but with HCA, researchers can measure synaptic function in primary neurons. The data can be used to discern biological properties of neurons, and results can be reported at the level of individual neurons and at whole-well and subpopulation levels.

For stem cell research, HCA can improve cell isolation, recovery, differentiation, and manipulation. Assays of human stem cells generate results that more closely mimic native conditions and lead to more physiologically- and pharmacologically-relevant conclusions.

Cytotoxicity assays of physiologically-relevant cells also generate results that more closely reflect the effects that would be seen in a living organism. HCA, then, is an exceptionally high- value approach when the effects in question are related to compound, drug, or safety.

Molecular Devices has developed a complete, integrated portfolio of HCA systems and software that can improve the productivity and success of drug discovery programs.

ImageXpress® Micro Widefield System

ImageXpress® Ultra Confocal System

ImageXpress® Velos Laser-Scanning Cytometer

MetaXpress® Analysis Software

MetaXpress® Parallel Processing Software

AcuityXpress™ Informatics Software

MDCStore™ Data Management Solution